Product Name: PFTAIRE2-CT
Product Number: AB-NK004-3
Size: | 25 µg | | Price: | 89.00 |
| | | $US | |
Target Full Name: Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 7 protein
Target Alias: A2S7; AL2S7; ALS2CR7; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 7 protein; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7; CDK15; PFTAIRE protein kinase 2; PFTK2; Serine/threonine-protein kinase ALS2CR7
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK004-2
Antibody Target Type: Pan-specific
Protein UniProt: Q96Q40 Protein SigNET: Q96Q40 Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PFTAIRE2 (ALS2CR7) sequence peptide Cat. No.: PE-01BEP80
Antibody Immunogen Sequence: CDLLASYQKGHHPAQFSK
Antibody Immunogen Description: Corresponds to amino acid residues C416 to K433; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-50 kDa.
Related Product 1: PFTAIRE2-CT blocking peptide Related Product 2: PFTAIRE1-ANT pan-specific antibody (Cat. No.: AB-NK287-1) Scientific Background: PFTAIRE2 (CDK15, PFTK2, ALS2CR7) is a protein-serine/threonine kinase of the CMGC group and CDK family. It controls cell cycle progression. PFTAIRE2 activity is mediated via a cyclin. PFTAIRE2 attenuates TRAIL induced apoptosis in part by phosphorylation of survivin at T34.
Figure 1. Dot blotting PFTAIRE2-CT antibody with recombinant purified proteins.