Product Name: AurKC-2
Product Number: AB-NK009-3
Size: 25 µg      Price:89.00
Target Full Name: Aurora Kinase C (serine/threonine-protein kinase 13)

Target Alias: AIE1; AIE2; AIK3; AurkC; Aurora,Ipl1,Eg2 protein 1 and 2; Aurora,Ipl1-related kinase 3; Aurora-C; STK13; STKD

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK009-3

Antibody Target Type: Pan-specific

Protein UniProt: Q9UQB9

Protein SigNET: Q9UQB9

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human AurKC (Aurora C, AIK3) sequence peptide Cat. No.: PE-01AWZ90

Antibody Immunogen Sequence: CSPAMRRLTVDDFEIG

Antibody Immunogen Description: Corresponds to amino acid residues S32 to G46; Pre-kinase catalytic domain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.5 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in mouse muscle.

Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-40 kDa.

Antibody Specificity: High-very high

Antibody Cross Reactivity: No significant cross-reactive proteins detected in various human cancer cell lines.

Related Product 1: AurKC-2 blocking peptide

Related Product 2: AurKC-1 pan-specific antibody (Cat. No.: AB-NK009-2)

Related Product 3: AurKC-pS193 phosphosite-specific antibody (Cat. No.: AB-PK532)

Scientific Background: AurC (AurKC) is a protein-serine/threonine kinase of the Other group and AUR family. It serves an essential component of the chromosomal passenger complex (CPC), which functions to ensure correct chromosomal alignment and segregation during mitosis. In addition, this protein complex is necessary for chromatin-induced microtubule stabilization and formation of the spindle apparatus. AurC has also been shown to play a key role in meiosis, specifically during spermatogenesis (production of sperm). Several mutations in the AurC gene have been observed in male patients with spermatogenic failure 5, including a 1-bp deletion (144delC) leading to premature translation termination at position 71 and a truncated protein without a catalytic domain, a C229Y substitution mutation in the kinase catalytic domain of the protein, and a 2A-to-G transition mutation at the splice site of intron 4 (436-2A-G) leading to the omission of exon 5 from the protein. These mutations result in a loss-of-function for the protein catalytic activity, therefore defects in AurC activity are thought to be the primary contributing factor to the development of spermatogenic failure 5.