Product Name: Erk1/2
Product Number: AB-NK055/56-2
Size: 25 µl      Price:89.00
      $US
Target Full Name: Mitogen-activated protein kinase 3

Target Alias: ERK1; ERK2; ERT1; ERT2; MAP kinase1; MAP kinase2; MAPK1; MAPK2; MAPK3; p38; p40; p41; p41mapk; p42 MAPK; p44 ERK1; p44 MAPK; PRKM1 PRKM2; PRKM3

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK055/56-2

Antibody Target Type: Pan-specific

Protein UniProt: P27361

Protein SigNET: P27361

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Production Method: Rabbit antiserum

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Storage Buffer: Rabbit antiserum

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting, Immunohistochemistry, ICC, Immunofluorescence

Antibody Dilution Recommended: 1:5000 (ECL) (WB), 1:50 (IHC), 10µg/mL (Flow cytometry)

Antibody Potency: Very high potency. Detects ~44 kDa (ERK1) and ~42 kDa (ERK2) proteins in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus

Antibody Positive Control: 1:5000 (ECL) (WB), 1:50 (IHC), 10 µg/ml (Flow cytometry)

Antibody Specificity: Very high

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Scientific Background: The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
Figure 1. Immunoblotting of various cell lines with AB-NK055-2 antibody at 1000X dilution. The target proteins ERK1 and ERK2 are indicated. Each lane was loaded with 15 µg of cell lysate protein. Max signal count was 65535


Figure 2. Immunoblotting of various tissue lines with AB-NK055-2 antibody at 1000X dilution. The target proteins ERK1 and ERK2 are indicated. Each lane was loaded with 15 µg of tissue lysate protein. Max signal count was 65531.


References

[1] Boulton TG. et al. (1991) Biochemistry. 30(1):278-86.

[2] Yoon S., and Seger R. (2006) Growth Factors 24:21-44.

[3] Wolf G. (2005) Antioxid Redox Signal 7:1337-1345.

[4] Chuerland D., Marmor G., Shainskaya A. and Seger R. (2008) J Biol Chem. Epub: http://www.jbc.org/cgi/doi/10.1074/jbc.M709030200.

[5] Zhuang S., and Schnellmann R.G. (2006) J Pharmacol Exp Ther 319:991-997.