Product Name: GRK (pan)
Product Number: AB-NK067-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: G protein-coupled receptor kinase 5
Target Alias: G protein-coupled receptor kinase; Pan-GRK; GRK-pan; GRK
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK067-2
Antibody Target Type: Pan-specific
Protein UniProt: P34947
Protein SigNET: P34947
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: G protein-coupled receptor kinase; Pan-GRK; GRK-pan; GRK
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK067-2
Antibody Target Type: Pan-specific
Protein UniProt: P34947
Protein SigNET: P34947
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5. 91% identical to human, and 99% identical to rat. >55% identity with GRK6 and GRK4. >40% identity with GRK7 and RK.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a 70 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-449 was sufficient for detection of GRK (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Cross-reacts with GRK6.
Related Product 1: GRK1Subtide - RHOK (GRK1) protein kinase substrate peptide
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a 70 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-449 was sufficient for detection of GRK (pan) in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: Cross-reacts with GRK6.
Related Product 1: GRK1Subtide - RHOK (GRK1) protein kinase substrate peptide
Related Product 2: GRKtide KinSub - GRK protein kinase substrate peptide
Related Product 3: GrkSubtide - BARK1 (GRK2; ADRBK1) protein kinase substrate peptide
Related Product 3: GrkSubtide - BARK1 (GRK2; ADRBK1) protein kinase substrate peptide
Scientific Background: G protein-coupled receptor kinases (GRK) are serine/threonine kinases that phosphorylate a variety of G-protein-coupled receptors (GPCRs), specifically activated forms, including adrenergic receptors, muscarinic acetylcholine receptors, dopamine receptors and opioid receptors. GRK receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation.
© Kinexus Bioinformatics Corporation 2017