Target Full Name: Janus protein-tyrosine kinase 1

Target Alias: JAK1A; Janus kinase 1; JAK1B; CE2.7.10.2; JAK-1; JTK3; JAK1_ENST00000342505; CCDS41346; Q0VGA4; Q4LDX3; Q6P669; Q8IV82; ENSG00000162434

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK084-5

Antibody Target Type: Pan-specific

Protein UniProt: P23458

Protein SigNET: P23458

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human JAK1 sequence peptide Cat. No.: PE-01AUO80

Antibody Immunogen Sequence: CFQNLIEGFEALLK

Antibody Immunogen Description: Corresponds to amino acid residues F1142 to K1154; Kinase last alpha-chain+C-terminus

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with recombinant human JAK1 on protein dot blots.