Target Full Name: MAPK/ERK protein-serine kinase 2 (MKK2); Dual specificity mitogen-activated protein kinase kinase 2

Target Alias: ERK activator kinase 2; MAP kinase kinase 2; MAP2K2; MAPK,ERK kinase 2; MAPKK2; MKK2; MP2K2; PRKMK2EC; MEK2; PRKMK2; CCDS12120.1; ENSG00000126934

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK100-3

Antibody Target Type: Pan-specific

Protein UniProt: P36507

Protein SigNET: P36507

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human MEK2 (MKK2, MAP2K2) sequence peptide

Antibody Immunogen Sequence: MLARRKPVLPALTC

Antibody Immunogen Description: Corresponds to amino acid residues M1 to T13; N-terminus

Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: 100 mM Tris-glycine, pH 7.0

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting