Product Name: MKK4-4 (MEK4-XI)
Product Number: AB-NK103-7
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: MAPK/ERK protein-serine kinase 4 (MKK4); Dual specificity mitogen-activated protein kinase kinase 4
Target Alias: c-Jun N- terminal kinase kinase 1; C-JUN N-terminal kinase kinase 1; JNK activating kinase 1; JNK kinase 1; JNKK1; Kinase SEK1; MAP2K4; SEK1; SERK1; SAPKK1; SAPKK-1; SKK1; CCDS11162.1; ENSG00000065559
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK103-7
Antibody Target Type: Pan-specific
Protein UniProt: P45985
Protein SigNET: P45985
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Target Alias: c-Jun N- terminal kinase kinase 1; C-JUN N-terminal kinase kinase 1; JNK activating kinase 1; JNK kinase 1; JNKK1; Kinase SEK1; MAP2K4; SEK1; SERK1; SAPKK1; SAPKK-1; SKK1; CCDS11162.1; ENSG00000065559
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK103-7
Antibody Target Type: Pan-specific
Protein UniProt: P45985
Protein SigNET: P45985
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MKK4 (MAP2K4, MEK4) sequence peptide
Antibody Immunogen Sequence: CLTKDESKRPKYKELLK
Antibody Immunogen Description: Corresponds to amino acid residues C347 to K363; Kinase catalytic subdomain 11
Antibody Immunogen Sequence: CLTKDESKRPKYKELLK
Antibody Immunogen Description: Corresponds to amino acid residues C347 to K363; Kinase catalytic subdomain 11
Production Method: The immunizing peptide was produced on an A.B.I. automated peptide synthesizer. The peptide was cleaved from the synthesis resin with HF and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by amino acid analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: 100 mM Tris-glycine, pH 7.0
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity on Western blots with recombinant Xenopus MEK2 (which is MKK4) expressed in E. coli.
Antibody Species Reactivity: Human; Mouse; Frog
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months, but either 0.1% sodium azide or 0.05% Thimerasol should be added. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity on Western blots with recombinant Xenopus MEK2 (which is MKK4) expressed in E. coli.
Antibody Species Reactivity: Human; Mouse; Frog
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 42-46 kDa.
Related Product 1: MKK4-1 pan-specific antibody (Cat. No.: AB-NK103-4)
Related Product 2: MKK4-2 pan-specific antibody (Cat. No.: AB-NK103-5)
Related Product 3: MKK4-3 pan-specific antibody (Cat. No.: AB-NK103-6)
Related Product 4: MKK4-pS257 phosphosite-specific antibody (Cat. No.: AB-PK715)
Related Product 5: MKK4-pS80 phosphosite-specific antibody (Cat. No.: AB-PK716)
Related Product 1: MKK4-1 pan-specific antibody (Cat. No.: AB-NK103-4)
Related Product 2: MKK4-2 pan-specific antibody (Cat. No.: AB-NK103-5)
Related Product 3: MKK4-3 pan-specific antibody (Cat. No.: AB-NK103-6)
Related Product 4: MKK4-pS257 phosphosite-specific antibody (Cat. No.: AB-PK715)
Related Product 5: MKK4-pS80 phosphosite-specific antibody (Cat. No.: AB-PK716)
Scientific Background: MKK4 (MAP2K4, MEK4) is a dual specificity protein kinase of the STE group and STE7 family Phosphorylation of S257 and T261 increases phosphotransferase activity. Phosphorylation of S80 inhibits its phosphotransferase activity. It acts as a critical component in the MAP kinase (MAPK) and the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signalling pathways. It has a role in maintaining peripheral lymphoid homeostasis and apoptosis. Mutations and deletions of this genes leads to dysregulated JNK signalling and promotes oncogenesis. MKK4 appears to be a tumour suppressor protein (TSP), but it may also promote metastasis. Cancer-related mutations in human tumours point to a loss of function of the protein kinase. The active form of the protein kinase normally acts to inhibit tumour cell proliferation. However, significantly elevated expression of MKK4 has been observed in invasive pancreatic cancer specimens, indicating a possible role for the protein in tumourigenesis. MKK4 over-expression is correlated with increased expression of heat shock protein 27 (HSP27) and matrix metalloproteinase 2 (MMP-2) protein, which are required for the increased invasive phenotype of the cells. Therefore, MKK4 appears to have an important role in the promotion of metastasis in pancreatic cancer cells. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.
© Kinexus Bioinformatics Corporation 2017