Product Name: p38d-3
Product Number: AB-NK121-4
Size: 25 µg      Price:89.00
      $US
Target Full Name: Mitogen-activated protein-serine kinase p38 delta; Mitogen-activated protein kinase 13

Target Alias: MAP kinase p38 delta; MAPK13; Mitogen-activated protein kinase p38 delta; MK13; P38 MAPK-delta;p38delta; PRKM13; Stress-activated protein kinase-4; SAPK4; p38d MAPK; ENSG00000156711

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK121-4

Antibody Target Type: Pan-specific

Protein UniProt: O15264

Protein SigNET: O15264

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human p38d MAPK (MAPK13) sequence peptide Cat. No.: PE-01AYI90

Antibody Immunogen Sequence: CPIARKDSRRRSGMKL

Antibody Immunogen Description: Corresponds to amino acid residues P351 to L365; C-terminus

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.75 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 1 µg/ml for immunoblotting

Antibody Potency: Medium immunoreactivity with recombinant human p38d on protein dot blots.

Antibody Species Reactivity: Human; Mouse

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 37-45 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human p38g and p38b, and no immunoreactivity with p38a.

Related Product 1: p38d-3 blocking peptide

Related Product 2: p38 pan-specific antibody (Cat. No.: AB-NK120-5)

Related Product 3: p38d-1 pan-specific antibody (Cat. No.: AB-NK121-2)

Related Product 4: p38d-2 pan-specific antibody (Cat. No.: AB-NK121-3)

Related Product 5: p38d-pT180+pY182 phosphosite-specific antibody (Cat. No.: AB-PK742)

Related Product 6: p38d-pY182 phosphosite-specific antibody (Cat. No.: AB-PK743)

Scientific Background: p38d (MAPK13) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is essential for MAP kinase signalling that can be induced via physical stress or proinflammatory cytokines. It is activated by phosphorylation at Y182 and probably T180 by (MKK3 (MAP2K3) and MKK6 (MAP2K6). p38d signalling can be induced via physical stress or proinflammatory cytokines resulting in the activation of the transcription factors ELK1 and ATF2. It can affect proliferation, differentiation, transcription regulation, translation regulation, cytoskeleton regulation, insulin secretion, cell survival and development. p38d can be a positive or negative regulator of the above processes depending on the cell context. Increased expression of p38d has been associated with increases proliferation and metastatic progression. It is also hypermethylated in some cancer cell lines. The rare liver cancer Cholangiocarcinoma (CCA, CC) has been noted to have a significant up-regulation of p38d compared to normal bile duct tissue. Insulin secretion and pancreatic beta cell survival can be regulated by p38d.
Figure 1. Dot blotting p38d-3 antibody with recombinant purified proteins.