Product Name: PAK6-APBD
Product Number: AB-NK124-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: Protein-serine/threonine kinase PAK 6

Target Alias: PAK5; KIAA1264; P21 protein (Cdc42/Rac)-activated kinase 7; P21-activated kinase 7; PAK 7; PAK-5; PAK7; PAK-7; MGC26232; RP5-1119D9_3; ENSG00000101349; KIAA1264KIAA1264; P21 protein (Cdc42/Rac)-activated kinase 7; P21-activated kinase 7; PAK 7; PAK-5; PAK7; PAK-7; MGC26232; RP5-1119D9_3; ENSG00000101349; KIAA1264

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK124-2

Antibody Target Type: Pan-specific

Protein UniProt: Q9NQU5

Protein SigNET: Q9NQU5

Antibody Type: Polyclonal

Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PAK6 sequence peptide Cat. No.: PE-01BEH90

Antibody Immunogen Sequence: CGSAMPVDGYISGLLNDIQK

Antibody Immunogen Description: Corresponds to amino acid residues G73 to K91; Post-PBD

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Very strong immunoreactivity of a target-sized protein by Western blotting in various mouse brain.

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 70-74 kDa.

Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactive proteins detected in diverse mouse tissues, except for a ~65 kDa protein in mouse testes, and weaker 50 and 37 kDa proteins in mouse brain, skeletal muscle and thymus. No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for a strong 38 kDa proten in A431 cells.

Related Product 1: PAK6-APBD blocking peptide

Scientific Background: PAK6 (PAK5) is a protein-serine/threonine kinase of the STE group and STE20 family. It can regulate gene transcription. MAPKK6/MAP2K6 or AR can induce PAK6 activity which will induce phosphorylation of the DNA-binding domain on an androgen receptor (AR) protein. The activation of PAK6 through MAP2K6/MAPKK6 can be inhibited through the three mutations S165A, S560A, and Y566F. It plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. It phosphorylates the DNA-binding domain of androgen receptor/AR and inhibits AR-mediated transcription. It also inhibits ESR1-mediated transcription. It may play a role in cytoskeleton regulation by interacting with IQGAP1. It can protect cells from apoptosis through phosphorylation of BAD. PAK6 suppresses tumour progression through regulation of androgen receptor homeostasis.