Product Name: PKD3-BKCD
Product Number: AB-NK139-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Protein-serine kinase C nu; Serine/threonine-protein kinase D3
Target Alias: EPK2; KPCD3; NPKC-nu; PRKCN; PRKD3; Protein kinase C, nu type; Protein kinase D3; Protein kinase EPK2
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK139-2
Antibody Target Type: Pan-specific
Protein UniProt: O94806
Protein SigNET: O94806
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKD3 (PRKCN) sequence peptide Cat. No.: PE-01BEU80
Target Alias: EPK2; KPCD3; NPKC-nu; PRKCN; PRKD3; Protein kinase C, nu type; Protein kinase D3; Protein kinase EPK2
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK139-2
Antibody Target Type: Pan-specific
Protein UniProt: O94806
Protein SigNET: O94806
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKD3 (PRKCN) sequence peptide Cat. No.: PE-01BEU80
Antibody Immunogen Sequence: TDTMVYFVGENNGDSSHNGC
Antibody Immunogen Description: Corresponds to amino acid residues T490 to N507; Just before kinase catalytic domain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues T490 to N507; Just before kinase catalytic domain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-105 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells or in mouse brain, skeletal muscle, testes and thymus.
Related Product 1: PKD3-BKCD blocking peptide
Related Product 2: CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Related Product 3: CREB1 (123-135) KinSub, biotinyl. - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells or in mouse brain, skeletal muscle, testes and thymus.
Related Product 1: PKD3-BKCD blocking peptide
Related Product 2: CREB1 (123-135) KinSub - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Related Product 3: CREB1 (123-135) KinSub, biotinyl. - cyclic-AMP response element binding protein-1 (K123-R135, human) peptide; Crebtide protein kinase substrate peptide
Scientific Background: PKD3 (PRKD3, PKC-nu and PKCv) is a protein-serine/threonine kinase of the CAMK group and PKD family. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord where it is poorly expressed. PKD3 is activated by DAG and phorbol esters, which mediates translocation to the cell membrane. PKD3 is also activated by phosphorylation at S731 and S735. PKD3 has been linked with the development of glioblastoma multiforme (GM). Mutations of the PKD3 gene were detected in some cases of chronic lymphocytic leukemia. It can also sensitize the effects of Raf kinase inhibitors in melanoma cells. It may be involve in the metastasis of lung cancer and contribute to prostate cancer cell growth.
© Kinexus Bioinformatics Corporation 2017