Product Name: Plk3-AKCD
Product Number: AB-NK147-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: Polo-like protein-serine kinase 3 (cytokine- inducible kinase (CNK))

Target Alias: CNK; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; FNK; Kinase Plk3; Plk-3; Polo-like kinase 3; PRK; Proliferation-related kinase; RP11-269F19_6; CCDS515.1; ENSG00000173846

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK147-2

Antibody Target Type: Pan-specific

Protein UniProt: Q9H4B4

Protein SigNET: Q9H4B4

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human Plk3 (CNK) sequence peptide Cat. No.: PE-01BFA75

Antibody Immunogen Sequence: KSKNHAQERDEVSGLVSGC

Antibody Immunogen Description: Corresponds to amino acid residues K357 to G374; After catalytic domain

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 0.18 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Weak immunoreactivity of a target-sized protein by Western blotting in A431 cells

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 72-78 kDa.

Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells. No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus.

Related Product 1: Plk3-AKCD blocking peptide

Related Product 2: Plk3Subtide - Plk3 (CNK) protein kinase substrate peptide

Scientific Background: Plk3 (CNK) is a protein-serine/threonine kinase of the Other group and Plk family. It plays a role in cell cycle regulation and tumourigenesis. Overexpression of Plk3 leads to mitotic arrest, cytokinesis defects, and apoptosis. Polymorphisms of Plk3 gene have been identified in human lung carcinoma cell lines.