Product Name: AurKB-3
Product Number: AB-NK193-4
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Aurora Kinase B (serine/threonine protein kinase 12)
Target Alias: AIK2; AIM1; AIM-1; ARK2; AurkB; Aurora,IPL1-related kinase 2; Aurora-2; Aurora-B; STK12; AurkB; IPL1; STK12; STK5; CCDS11134.1; ENSG00000178999
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK193-4
Antibody Target Type: Pan-specific
Protein UniProt: Q96GD4
Protein SigNET: Q96GD4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human AurKB (Aurora B, AIM-1) sequence peptide Cat. No.: PE-01AWX85
Target Alias: AIK2; AIM1; AIM-1; ARK2; AurkB; Aurora,IPL1-related kinase 2; Aurora-2; Aurora-B; STK12; AurkB; IPL1; STK12; STK5; CCDS11134.1; ENSG00000178999
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK193-4
Antibody Target Type: Pan-specific
Protein UniProt: Q96GD4
Protein SigNET: Q96GD4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human AurKB (Aurora B, AIM-1) sequence peptide Cat. No.: PE-01AWX85
Antibody Immunogen Sequence: CAQVSAHPWVRANSRR
Antibody Immunogen Description: Corresponds to amino acid residues A319 to R333; Kinase last alpha-chain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues A319 to R333; Kinase last alpha-chain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with recombinant human AurKB on protein dot blots.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with recombinant human AurKB on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-45 kDa.
Antibody Cross Reactivity: Very strong immunoreactivity on protein dot blots with recombinant human AurKA, and no immunoreactivity with recombinant human AurKC.
Related Product 1: AurKB-3 blocking peptide
Related Product 2: AurKB pan-specific antibody (Cat. No.: AB-NK193-2P)
Related Product 3: AurKB-1 pan-specific antibody (Cat. No.: AB-NK193-2)
Related Product 4: AurKB-2 pan-specific antibody (Cat. No.: AB-NK193-3)
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 35-45 kDa.
Antibody Cross Reactivity: Very strong immunoreactivity on protein dot blots with recombinant human AurKA, and no immunoreactivity with recombinant human AurKC.
Related Product 1: AurKB-3 blocking peptide
Related Product 2: AurKB pan-specific antibody (Cat. No.: AB-NK193-2P)
Related Product 3: AurKB-1 pan-specific antibody (Cat. No.: AB-NK193-2)
Related Product 4: AurKB-2 pan-specific antibody (Cat. No.: AB-NK193-3)
Related Product 5: AurKB-pS227 phosphosite-specific antibody (Cat. No.: AB-PK530)
Related Product 6: AurKB-pT232 phosphosite-specific antibody (Cat. No.: AB-PK531)
Related Product 6: AurKB-pT232 phosphosite-specific antibody (Cat. No.: AB-PK531)
Scientific Background: AurB (AurKB, AIK2) is a protein-serine/threonine kinase of the Other group and AUR family. It serves as a component of the chromosomal passenger complex (CPC) that plays a critical role in regulating mitosis. AurB may be a tumour requiring protein (TRP). Its low rate of mutation and down-regulation in human cancers supports it identification as a tumour-requiring protein, and as a target for cancer drug development. However, AurB is overexpressed in some colorectal cancers and other tumour cell lines, which was thought to lead to multinuclearity, increased ploidy, and a predisposition to cancer. Through its dominant-negative effect on cytokinesis, disruptive regulation of its expression is potentially responsible for the perturbing the chromosomal integrity in cancer cells. A K106R mutation leads to loss of phosphotransferase activity, and severely impairs mitosis.
Figure 1. Dot blotting AurKB-3 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017