Product Name: ERK5-2
Product Number: AB-NK206-4
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Extracellular regulated protein-serine kinase 5 (Big MAP kinase 1 (BMK1))
Target Alias: BMK; BMK1; BMK1 kinase; ERK4; ERK-5; Extracellular-signal-regulated kinase 5; Kinase ERK5; MAPK7; PRKM7; ENSG00000166484
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK206-4
Antibody Target Type: Pan-specific
Protein UniProt: Q13164
Protein SigNET: Q13164
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK5 (MAPK7) sequence peptide Cat. No.: PE-01AXI95
Target Alias: BMK; BMK1; BMK1 kinase; ERK4; ERK-5; Extracellular-signal-regulated kinase 5; Kinase ERK5; MAPK7; PRKM7; ENSG00000166484
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK206-4
Antibody Target Type: Pan-specific
Protein UniProt: Q13164
Protein SigNET: Q13164
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ERK5 (MAPK7) sequence peptide Cat. No.: PE-01AXI95
Antibody Immunogen Sequence: CTRERIKEAIVAEIED
Antibody Immunogen Description: Corresponds to amino acid residues T373 to D387; Post-kinase last alpha-chain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues T373 to D387; Post-kinase last alpha-chain
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human ERK5 on protein dot blots.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human ERK5 on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 88-115 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human ERK1 and ERK2.
Related Product 1: ERK5-2 blocking peptide
Related Product 2: ERK5-3 pan-specific antibody (Cat. No.: AB-NK206-5)
Related Product 3: ERK5-NT (ERK5-4) pan-specific antibody (Cat. No.: AB-NK206-2)
Related Product 4: ERK5-pT219+pY221 phosphosite-specific antibody (Cat. No.: AB-PK625)
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 88-115 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human ERK1 and ERK2.
Related Product 1: ERK5-2 blocking peptide
Related Product 2: ERK5-3 pan-specific antibody (Cat. No.: AB-NK206-5)
Related Product 3: ERK5-NT (ERK5-4) pan-specific antibody (Cat. No.: AB-NK206-2)
Related Product 4: ERK5-pT219+pY221 phosphosite-specific antibody (Cat. No.: AB-PK625)
Related Product 5: ERK5-pY221 phosphosite-specific antibody (Cat. No.: AB-PK626)
Related Product 6: ERK5 -PNT (ERK5-5) pan-specific antibody (Cat. No.: AB-NK206-1)
Related Product 6: ERK5 -PNT (ERK5-5) pan-specific antibody (Cat. No.: AB-NK206-1)
Scientific Background: ERK5 (MAPK7) is a protein-serine/threonine kinase of the CMGC group and MAPK family. It is important in controlling a cell's proliferation, differentiation, and cell survival. Phosphorylation of T219 and Y221 by MEK5 (MAP2K5) increases the phosphotransferase activity of ERK5. MEK5 is activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF). ERK5 may be a tumour requiring protein (TRP). Although there has been no direct linkage of ERK5 to formation of carcinomas in humans in vivo, in vitro ERK5 over-expression has been shown to increase metastasis, and ERK5 inhibition has been shown to inhibit osteosarcoma cell motility. Upregulation of the MEK5 gene has been noted in breast, prostate, and liver cancer.
Figure 1. Dot blotting ERK5-2 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017