Product Name: CSF1R
Product Number: AB-NK234-2P
Size: 25 µg      Price:89.00
      $US
Target Full Name: Macrophage colony-stimulating factor 1 receptor

Target Alias: CD115; C-fms; Colony stimulating factor 1 receptor; CSF-1-R; CSFMR; FMS; Fms proto-oncogene; Kinase CSFR; Macrophage colony stimulating factor I receptor precursor; M-CSFR; FIM2; FIM2; CCDS4302.1; A2VDG3; Q6LEI2; ENSG00000182578

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK234-2P

Antibody Target Type: Pan-specific

Protein UniProt: P07333

Protein SigNET: P07333

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human CSF1R (Fms) sequence peptide Cat. No.: PE-01ATQ99

Antibody Immunogen Sequence: CALTLVPAELVRIRGE

Antibody Immunogen Description: Corresponds to amino acid residues A204 to E218

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Potency: Weak reactivity with recombinant human CSF1R on protein dot blots.

Antibody Species Reactivity: Human; Mouse; Rat

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human Flt3 and Kit.

Related Product 1: CSF1R blocking peptide

Related Product 2: CSF1R pan-specific antibody (Cat. No.: AB-NK234-1)

Related Product 3: CSF1R-pS807+pY809 phosphosite-specific antibody (Cat. No.: AB-PK586)

Related Product 4: CSF1R-pY699 phosphosite-specific antibody (Cat. No.: AB-PK587)

Related Product 5: CSF1R-pY809 phosphosite-specific antibody (Cat. No.: AB-PK588)

Related Product 6: CSF1R -1 pan-specific antibody (Cat. No.: AB-NK234-3)

Scientific Background: CSF1R (Fms) is a member of the TK group of protein kinases in the PDGFR family. It acts as a membrane receptor for macrophage colony-stimulating factor 1 (CSF1) and IL34, and it functions in the regulation of cell survival, proliferation, and differentiation in hematopoietic precursor cells, specifically monocytes and macrophages. However, this kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord, where it is more moderately expressed. Orthologues of CSF1R are highly conserved in mammals and birds. CSF1R contains a so-called kinase insert domain and is a member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in CSF1R have been associated with providing sustained signals for cell growth and a predisposition to myeloid malignancy. CSF1R has been linked with the development of acute myeloid leukemia, chronic myelomonocytic leukemia, myelodysplasia, hepatocellular carcinomas, breast cancer, and lung squamous cell carcinomas (LSCC). Gain-of-function mutations in the Fms gene are associated with several forms of cancer.