Product Name: WNK2-2
Product Number: AB-NK253-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Serine/threonine-protein kinase WNK2
Target Alias: Antigen NY-CO-43; KIAA1760; NY-CO-43; P,OKcl.13; P/OKcl.13; PRKWNK2; Protein kinase with no lysine 2; Protein kinase, lysine-deficient 2; WNK lysine deficient protein kinase 2; SDCCAG43; KAIA302; ENSG00000165238
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK253-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y3S1
Protein SigNET: Q9Y3S1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK2 sequence peptide Cat. No.: PE-01AZC95
Target Alias: Antigen NY-CO-43; KIAA1760; NY-CO-43; P,OKcl.13; P/OKcl.13; PRKWNK2; Protein kinase with no lysine 2; Protein kinase, lysine-deficient 2; WNK lysine deficient protein kinase 2; SDCCAG43; KAIA302; ENSG00000165238
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK253-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y3S1
Protein SigNET: Q9Y3S1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK2 sequence peptide Cat. No.: PE-01AZC95
Antibody Immunogen Sequence: CDEDDLKAVATSLDGR
Antibody Immunogen Description: Corresponds to amino acid residues D179 to R193
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues D179 to R193
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.34mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Concentration: 0.34mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 250-300 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK2-2 blocking peptide
Related Product 2: WNK2-1 pan-specific antibody (Cat. No.: AB-NK253-1)
Related Product 3: WNK2-3 pan-specific antibody (Cat. No.: AB-NK253-3)
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK2-2 blocking peptide
Related Product 2: WNK2-1 pan-specific antibody (Cat. No.: AB-NK253-1)
Related Product 3: WNK2-3 pan-specific antibody (Cat. No.: AB-NK253-3)
Scientific Background: WNK2 (PRKWNK2) is a protein-serine/threonine kinase of the Other group and WNK family. It plays a critical role in the regulation of electrolyte homeostasis, signalling survival, and proliferation. Activation requires autophosphorylation of S356. Phosphorylation of S352 also promotes increased phosphotransferase activity. It activates sodiu-coupled chloride cotransporters and inhibits potassium-coupled chloride cotransporters. It is also involved in reducing ERK1/2 activation. ERK1/2 expression can also increase through activation of of MEK1 via Rac1-mediate PAK1 activation. WNK2 may be a tumour suppressor protein (TSP). WNK2 is also called serologically defined colon cancer antigen 43. Loss of WNK2 expression was found in adult gliomas and triggers Rac1-mediated tumour cell invasiveness. Loss of expression of WNK2 is also found in meningioma and facilitates increased cell cycle progression and proliferation.
Figure 1. Dot blotting WNK2-2 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017