Product Name: WNK3-3
Product Number: AB-NK254-3
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Serine/threonine-protein kinase WNK3
Target Alias: KIAA1566; PRKWNK3; Protein kinase, lysine-deficient 3; RP3-390O13_2; CCDS14357.1; ENSG00000196632
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK254-3
Antibody Target Type: Pan-specific
Protein UniProt: Q9BYP7
Protein SigNET: Q9BYP7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK3 (PRKWNK3) sequence peptide Cat. No.: PE-01AZG99
Target Alias: KIAA1566; PRKWNK3; Protein kinase, lysine-deficient 3; RP3-390O13_2; CCDS14357.1; ENSG00000196632
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK254-3
Antibody Target Type: Pan-specific
Protein UniProt: Q9BYP7
Protein SigNET: Q9BYP7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK3 (PRKWNK3) sequence peptide Cat. No.: PE-01AZG99
Antibody Immunogen Sequence: CGPFQPGMNMQAFPTS
Antibody Immunogen Description: Corresponds to amino acid residues G1773 to S1787;
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Immunogen Description: Corresponds to amino acid residues G1773 to S1787;
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.26 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Concentration: 0.26 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 170-250 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK3-3 blocking peptide
Related Product 2: WNK3-1 pan-specific antibody (Cat. No.: AB-NK254-1)
Related Product 3: WNK3-2 pan-specific antibody (Cat. No.: AB-NK254-2)
Antibody Specificity: Medium
Antibody Cross Reactivity: Weak immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK3-3 blocking peptide
Related Product 2: WNK3-1 pan-specific antibody (Cat. No.: AB-NK254-1)
Related Product 3: WNK3-2 pan-specific antibody (Cat. No.: AB-NK254-2)
Scientific Background: WNK3 (PRKWNK2) is a protein-serine/threonine kinase of the Other group and WNK family. Activation requires autophosphorylation of S308. Phosphorylation of S304 also promotes increased activity. Interestingly, WNK3 lacks the catalytic lysine in subdomain II that is believed to be critical for phoshotransferase activity, and instead has a conserved lysine in subdomain I. It functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway.
Figure 1. Dot blotting WNK3-3 antibody with recombinant purified proteins.
© Kinexus Bioinformatics Corporation 2017