Product Name: HGK-NT
Product Number: AB-NK300-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Mitogen-activated protein kinase kinase kinase kinase 4

Target Alias: FLH21957; Hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK kinase kinase kinase 4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK; FLJ10410; FLJ20373; FLJ90111; KIAA0687; NIK; O95819; ENSG0000007105

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NK300-1

Antibody Target Type: Pan-specific

Protein UniProt: O95819

Protein SigNET: O95819

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human HGK (ZC1) sequence peptide Cat. No.: PE-01BFM

Antibody Immunogen Sequence: ANDSPAKSLVDIDLSSLRDC

Antibody Immunogen Description: Corresponds to amino acid residues A2 to D20; N-terminus

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 135-150 kDa.

Antibody Specificity: High

Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for very strong ~82 kDa and ~25-26 kDa cross-reactive proteins. No cross-reactivities detected in mouse tissues, except for very strong ~85 and ~90 kDa proteins in mouse brain, skeletal muscle and testes.

Related Product 1: HGK-NT blocking peptide

Related Product 2: HGK-pT187 phosphosite-specific antibody (Cat. No.: AB-PK653)

Scientific Background: ZC1 (HGK, MAP4K4) is a protein-serine/threonine kinase of the STE group and STE20 family. It may play a role in the response to environmental stress and cytokines such as TNF-alpha, and appears to act upstream of the JUN N-terminal pathway. ZC1 can phosphorylate SMAD1 at T322. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.