Product Name: ZC2-CT
Product Number: AB-NK301-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: TRAF2 and NCK-interacting kinase
Target Alias: TNIK; KIAA0551; TRAF2 and NCK interacting kinase
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK301-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UKE5
Protein SigNET: Q9UKE5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ZC2 (TNIK) sequence peptide Cat. No.: PE-01BFN, PE-01BFO
Antibody Immunogen Sequence: CGSSQVFFMTLNRNSMMNW
Target Alias: TNIK; KIAA0551; TRAF2 and NCK interacting kinase
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK301-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UKE5
Protein SigNET: Q9UKE5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ZC2 (TNIK) sequence peptide Cat. No.: PE-01BFN, PE-01BFO
Antibody Immunogen Sequence: CGSSQVFFMTLNRNSMMNW
Antibody Immunogen Description: Corresponds to amino acid residues G1343 to W1360; C-terminus
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 180-200 kDa.
Antibody Specificity: Very high
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 180-200 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells. No cross-reactivities detected in diverse mouse tissues, except for a weak ~50 kDa protein in mouse skeletal muscle and thymus.
Related Product 1: ZC2-CT blocking peptide
Related Product 1: ZC2-CT blocking peptide
Scientific Background: ZC2 (TNIK; TRAF2 and NCK-interacting protein kinase) is a protein-serine/threonine kinase of the STE group and STE20 family. It may be involved in cytoskeleton regulation. Mutations at the 54, 152-153 and 171-172 positions of ZC2 lead to loss of autophosphorylation. It acts as an essential activator of the Wnt signaling pathway, and is recruited to promoters of Wnt target genes and required to activate their expression. It may act by phosphorylating TCF4/TCF7L2, and appears to function upstream of the JUN N-terminal pathway. It may mediate part of the response to environmental stress. It is part of a signaling complex composed of NEDD4, RAP2A and TNIK, which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on T322.
© Kinexus Bioinformatics Corporation 2017