Product Type Specific: Biotin pan-specific antibody

Antibody Code: NM001-4

Antibody Target Type: Pan-specific

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human sequence peptide Cat. No.: PE-03AAZ65

Antibody Immunogen Sequence: Biotin-SEGC

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Antibody Specificity: High

Antibody Cross Reactivity: Strongly cross-reactive ~50 kDa protein detected in mouse muscle, a 75 kDa protein in mouse testes and most other tissues, and to a lesser extent a 150 kDa protein cross-reacted strongly in A431 and HepG2 cells.

Related Product 1: Biotin blocking peptide