Product Name: Erp57 (GRP58)
Product Number: AB-NN040-2
| Size: | 50 µg | Price: | 89.00 | |
| $US |
Target Full Name: Protein disulfide-isomerase A3
Target Alias: ERp60; ERp61; Grp57; Grp58; P58; PDIA3; PI PLC
Product Type Specific: Nuclear receptor pan-specific antibody
Antibody Code: NN040-2
Antibody Target Type: Pan-specific
Protein UniProt: P30101
Protein SigNET: P30101
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Human recombinant Erp57 (Grp58)
Production Method: Protein G purified
Target Alias: ERp60; ERp61; Grp57; Grp58; P58; PDIA3; PI PLC
Product Type Specific: Nuclear receptor pan-specific antibody
Antibody Code: NN040-2
Antibody Target Type: Pan-specific
Protein UniProt: P30101
Protein SigNET: P30101
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Human recombinant Erp57 (Grp58)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Detects a ~57 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Dog | Guinea pig | Hamster | Monkey | Pig | Rabbit
Antibody Positive Control: 0.5 µg/ml of SMC-168 was sufficient for detection of ERp57 in 10 µg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Dog | Guinea pig | Hamster | Monkey | Pig | Rabbit
Antibody Positive Control: 0.5 µg/ml of SMC-168 was sufficient for detection of ERp57 in 10 µg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding
domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).
References
[1] Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.
[2] Williams D.B. (2005) J Cell Sci. 119: 615-623
[3] Maattanen P. et al. (2006) Biochem Cell Biol. 84: 881-889.
[4] Oliver J.D. et al. (1999) Mol Bio Cell. 10: 2573-2582.
[5] Oliver J.D. et al. (1997) Science 275: 86-88.
[6] Solda T. et al. (2006) J Biol Chem 281: 6219-6226.
[7] Kimura T. et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.
[8] Chen, G. et al. (2002) Clin Cancer Res 8(7): 2298-2305.
[1] Herbert D.N. and Molinari M. (2007) Physiol Rev. 87: 1377-1408.
[2] Williams D.B. (2005) J Cell Sci. 119: 615-623
[3] Maattanen P. et al. (2006) Biochem Cell Biol. 84: 881-889.
[4] Oliver J.D. et al. (1999) Mol Bio Cell. 10: 2573-2582.
[5] Oliver J.D. et al. (1997) Science 275: 86-88.
[6] Solda T. et al. (2006) J Biol Chem 281: 6219-6226.
[7] Kimura T. et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230.
[8] Chen, G. et al. (2002) Clin Cancer Res 8(7): 2298-2305.
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