Product Name: GRP94
Product Number: AB-NN049-3
Size: 50 µg      Price:89.00
      $US
Target Full Name: Endoplasmin

Target Alias: HSP90B1; GP96; TRA1; ECGP

Product Type Specific: Heat shock/stress protein pan-specific antibody

Antibody Code: NN049-3

Antibody Target Type: Pan-specific

Protein UniProt: P14625

Protein SigNET: P14625

Antibody Type: Monoclonal

Antibody Host Species: Rat

Antibody Ig Isotype Clone: IgG2a

Antibody Immunogen Source: Purified Grp94 isolated from chicken oviducts

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | ICC/Immunofluorescence | Immunoprecipitation | FCM

Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Weak potency. Detects a ~98 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Dog | Chicken | Guinea pig | Hamster | Hr | Monkey | Pig | Rabbit | Sheep | Xenopus

Antibody Positive Control: 0.5 µg/ml of SMC-105 was sufficient for detection of Grp94 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Antibody Cross Reactivity: Does not detect human HSP90, Grp74, or GrpE from E.coli.

Related Product 1: GRP94 pan-specific antibody (Cat. No.: AB-NN049-4)

Scientific Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2) Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
References

[1] Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128.

[2] Srivastava P.K. et al. (1994) Immunogenetics. 39(2):93-98.

[3] Mazzarella R.A., and Green M. (1987) J Biol Chem 262:8875-8883.

[4] Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9):5643-5649.

[5] Soldano K.L. et al. (2003) J Biol Chem 278(48): 48330-48338.

[6] Chu F. et al. (2006) Protein Sci 15(6): 1260-1269.

[7] Peter F. et al., (1992) J Biol Chem 267: 10631-10637.

[8] Allen S. et al. (2000) Blood 96(2): 560-568.

[9] Sato K et al. (2001) Blood 98(6): 1852-1857.

[10] Yun S.-W. et al (2000) Brain Research Bulletin.52(5):371-378.

[11] Choukhi A. et al. (1998) J. Virol. 72: 3851-3858.

[12] Hoshino T. et al. (1998) Blood 91(11): 4379-4386; Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104; Gusarova V. et al. (2001) J. Biol. Chem. 276(27): 24891-24900.