Product Name: HSP40, YDJ1
Product Number: AB-NN057-5
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Mitochondrial protein import protein MAS5
Target Alias: DNAJA2; CPR3; HIRIP4; DNAJ; DNJ3; DJ3; RDJ2; HIRa interacting protein4
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-5
Antibody Target Type: Pan-specific
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Full length protein yeast HSP40 (YDJ1)
Production Method: Protein G purified
Target Alias: DNAJA2; CPR3; HIRIP4; DNAJ; DNJ3; DJ3; RDJ2; HIRa interacting protein4
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-5
Antibody Target Type: Pan-specific
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Full length protein yeast HSP40 (YDJ1)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~40 kDa protein by Western blotting. Yeast specific product.
Antibody Concentration: 1 mg/ml
Storage Buffer: 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~40 kDa protein by Western blotting. Yeast specific product.
Antibody Species Reactivity: Yeast
Antibody Positive Control: 0.5 µg/ml of SMC-150 was sufficient for detection of 50ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross react with human, mouse or rat HSP40.
Related Product 1: HSP40 pan-specific antibody (Cat. No.: AB-NN057-3)
Related Product 2: HSP40 pan-specific antibody (Cat. No.: AB-NN057-4)
Related Product 3: HSP40, Hdj1 pan-specific antibody (Cat. No.: AB-NN057-2)
Related Product 4: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-6)
Antibody Positive Control: 0.5 µg/ml of SMC-150 was sufficient for detection of 50ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross react with human, mouse or rat HSP40.
Related Product 1: HSP40 pan-specific antibody (Cat. No.: AB-NN057-3)
Related Product 2: HSP40 pan-specific antibody (Cat. No.: AB-NN057-4)
Related Product 3: HSP40, Hdj1 pan-specific antibody (Cat. No.: AB-NN057-2)
Related Product 4: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-6)
Scientific Background: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not.
Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).
References
[1] Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.
[2] Fan C.Y. et al. (2003) Cell Stress Chaperones 8: 309–316.
[3] Terda K. et al. (1997) J Cell Biol. 139: 1089-1095.
[4] Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
[5] Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.
[6] Cyr D.M. et al. (1992) J Biol Chem. 267: 20927–20931.
[7] Höhfeld J. et al. (2001) EMBO Rep. 2: 885–890.
[1] Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.
[2] Fan C.Y. et al. (2003) Cell Stress Chaperones 8: 309–316.
[3] Terda K. et al. (1997) J Cell Biol. 139: 1089-1095.
[4] Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
[5] Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.
[6] Cyr D.M. et al. (1992) J Biol Chem. 267: 20927–20931.
[7] Höhfeld J. et al. (2001) EMBO Rep. 2: 885–890.
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