Product Name: p53
Product Number: AB-NN082-2
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Cellular tumour antigen p53
Target Alias: P53; LFS1; TRP53; FLJ92943; TP53; p53 Tumour Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumour protein p53
Product Type Specific: Transcription protein pan-specific antibody
Antibody Code: NN082-2
Antibody Target Type: Pan-specific
Protein UniProt: P04637
Protein SigNET: P04637
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein (Human), amino acids 46-53
Target Alias: P53; LFS1; TRP53; FLJ92943; TP53; p53 Tumour Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumour protein p53
Product Type Specific: Transcription protein pan-specific antibody
Antibody Code: NN082-2
Antibody Target Type: Pan-specific
Protein UniProt: P04637
Protein SigNET: P04637
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein (Human), amino acids 46-53
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation | ELISA | RIA | FCM
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation | ELISA | RIA | FCM
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~53 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human
Antibody Positive Control: 1 µg/ml of SMC-183 was sufficient for detection of p53 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: p53 pan-specific antibody (Cat. No.: AB-NN082-3)
Antibody Species Reactivity: Human
Antibody Positive Control: 1 µg/ml of SMC-183 was sufficient for detection of p53 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: p53 pan-specific antibody (Cat. No.: AB-NN082-3)
Scientific Background: The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).
References
[1] Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16(2): 133-9.
[2] Strachan T. et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics.
[3] Hansen J.E. et al. (2007) Cancer Research 67: 1769-1774.
[4] Di Cesare E. et al. (2001) Ann Clin Lab Sci 31: 253-8.
[5] Akere A., Otegbayo J.A. (2007) Singapore Med J. 48(1): 41-4.
[1] Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16(2): 133-9.
[2] Strachan T. et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics.
[3] Hansen J.E. et al. (2007) Cancer Research 67: 1769-1774.
[4] Di Cesare E. et al. (2001) Ann Clin Lab Sci 31: 253-8.
[5] Akere A., Otegbayo J.A. (2007) Singapore Med J. 48(1): 41-4.
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