Target Full Name: DNA polymerase delta auxiliary protein

Target Alias: cb16; MGC8367; PCNA_HUMAN; Polymerase delta accessory protein; Proliferating cell nuclear antigen; Mutagen-sensitive 209 protein; OTTHUMP00000030189; PCNAR; Pcna/cyclin; HGCN8729; wu:fb36g03; PCNA; ATLD2; fb36g03; OTTHUMP00000030190; Cyclin

Product Type Specific: DNA synthesis protein pan-specific antibody

Antibody Code: NN087-2

Antibody Target Type: Pan-specific

Protein UniProt: P12004

Protein SigNET: P12004

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Synthetic peptide of Human PCNA

Production Method: Peptide Affinity purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a weak ~37 kDa protein band in human 293 Trap cell lysates.

Antibody Species Reactivity: Human

Antibody Positive Control: A 1:1000 dilution of SPC-691 was sufficient for detection of PCNA in 15 µg of Human 293T cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and 293 Trap cell lysates.