Product Name: Nrf2
Product Number: AB-NN140-1
Size: 12.5 µg      Price:89.00
      $US
Target Full Name: Nuclear factor erythroid 2-related factor 2

Target Alias: NFE2L2; HEBP1; NRF2

Product Type Specific: Transcription protein pan-specific antibody

Antibody Code: NN140-1

Antibody Target Type: Pan-specific

Protein UniProt: Q16236

Protein SigNET: Q16236

Antibody Type: Polyclonal

Antibody Host Species: Goat

Antibody Immunogen Source: Peptide derived from human Nrf2 protein (a.a. 445-458)

Production Method: Affinity purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Storage Buffer: TBS, pH7.3, 0.02% sodium azide, 0.5% BSA

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: ELISA, Western blotting

Antibody Dilution Recommended: 1:500-1500 (WB), 1:64000 (ELISA)

Antibody Species Reactivity: Bovine, Canine, Porcine, Human, Rat, Mouse

Antibody Positive Control: A 1:1500 dilution of SPC-218 was sufficient for detection of Nrf2 in 35 µg of human lung lysate by electrochemiluminescence analysis using donkey anti-goat IgG:HRP as the secondary antibody.

Scientific Background: Nrf2 is a transcription factor which plays a role in the oxidative stress response. Under normal cellular conditions, it is sequestered in the cytoplasm by Keap1 and Cullin3. When under oxidative stress, Nrf2 is release from Keap1 by p62 and is translocated to the nucleus where it acts as a transcription factor for the expression of oxidative stress-related products.
References

[1] Itoh, K., Wakabayashi, N., Katoh, Y., Ishii, T., Igarashi, K., Engel, J. et al. (1999). Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain. Genes Dev, 13(1):76-86.