Product Name: Alpha A Crystallin
Product Number: AB-NN149-5
| Size: | 50 µg | Price: | 89.00 | |
| $US |
Target Full Name: Alpha A Crystallin
Target Alias: Heat shock protein beta4; Acry 1; CRYA1; CRYAA; HSPB4
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN149-5
Antibody Target Type: Pan-specific
Protein UniProt: P02489
Protein SigNET: P02489
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Native Alpha Crystallin
Production Method: Protein G purified
Target Alias: Heat shock protein beta4; Acry 1; CRYA1; CRYAA; HSPB4
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN149-5
Antibody Target Type: Pan-specific
Protein UniProt: P02489
Protein SigNET: P02489
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Native Alpha Crystallin
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ELISA
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~20 kDa protein in cell and tissue lysates by Western blotting.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ELISA
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~20 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine
Antibody Positive Control: 0.5 µg/ml was sufficient for detection of 100ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Related Product 1: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-1)
Related Product 2: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-2)
Related Product 3: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-3)
Antibody Positive Control: 0.5 µg/ml was sufficient for detection of 100ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Related Product 1: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-1)
Related Product 2: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-2)
Related Product 3: Alpha B Crystallin pan-specific antibody (Cat. No.: AB-NN149-3)
Scientific Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its’ phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).
© Kinexus Bioinformatics Corporation 2017