Product Name: KDEL Receptor
Product Number: AB-NN153
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: ER lumen protein-retaining receptor 1
Target Alias: ERD2; ERD2.1; ERD21; HDEL; KDEL; KDEL R1; KDELR1; PM23
Product Type Specific: Endoplasmic reticulum receptor pan-specific antibody
Antibody Code: NN153
Antibody Target Type: Pan-specific
Protein UniProt: P24390
Protein SigNET: P24390
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
Target Alias: ERD2; ERD2.1; ERD21; HDEL; KDEL; KDEL R1; KDELR1; PM23
Product Type Specific: Endoplasmic reticulum receptor pan-specific antibody
Antibody Code: NN153
Antibody Target Type: Pan-specific
Protein UniProt: P24390
Protein SigNET: P24390
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Detects a ~25 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Monkey | Hamster | Rabbit | Pig | Sheep | Dog | Chicken | Drosophila | Xenopus
Antibody Positive Control: 1 µg/ml was sufficient for detection of KDEL receptor in 20 µg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Antibody Specificity: Medium-high
Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Monkey | Hamster | Rabbit | Pig | Sheep | Dog | Chicken | Drosophila | Xenopus
Antibody Positive Control: 1 µg/ml was sufficient for detection of KDEL receptor in 20 µg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Antibody Specificity: Medium-high
Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4).
In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
References
[1] Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737.
[2] Forthoffer N. et al. (2002) J Bioenerg Biomemb 34(3): 209-219.
[3] Aoe T. et al. (1997) EMBO J. 16: 7305-7316.
[4] Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328.
[5] Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163.
[6] Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from http://cellbio.utmb.edu/cellbio/rer1.htm
[1] Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737.
[2] Forthoffer N. et al. (2002) J Bioenerg Biomemb 34(3): 209-219.
[3] Aoe T. et al. (1997) EMBO J. 16: 7305-7316.
[4] Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328.
[5] Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163.
[6] Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from http://cellbio.utmb.edu/cellbio/rer1.htm
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