Product Name: PDK4
Product Number: AB-NN178-3P
Size: 25 µg      Price:89.00
      $US
Target Full Name: Pyruvate dehydrogenase kinase isoform 4; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial

Target Alias: Pyruvate dehydrogenase kinase isoform 4; PDK4;

Product Type Specific: Protein kinase pan-specific antibody

Antibody Code: NN178-3P

Antibody Target Type: Pan-specific

Protein UniProt: Q16654

Protein SigNET: Q16654

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Human PDK4 (PDHK4) sequence peptide Cat. No.: PE-01ARK90

Antibody Immunogen Sequence: CDQYYLSSPELKLTQV

Antibody Immunogen Description: Corresponds to amino acid residues C215 to V230

Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human. Mouse; Rat

Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-55 kDa.

Antibody Specificity: Very high

Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human PDK1, PDK3 and PDK4.

Related Product 1: PDK4 blocking peptide

Related Product 2: PDK4 pan-specific antibody (Cat. No.: AB-NN178-1)

Related Product 3: PDK4 pan-specific antibody (Cat. No.: AB-NN178-2P)

Related Product 4: PDK4-1 pan-specific antibody (Cat. No.: AB-NN178-2)

Related Product 5: PDK4-3 pan-specific antibody (Cat. No.: AB-NN178-3)

Scientific Background: PDHK4 (PDK4, pyruvate dehydrogenase kinase 4) is a protein-serine/threonine kinase of the Atypical group and PDHK family. It phosphorylates and inactivates the enzyme pyruvate dehydrogenase. Deficiency of PDHK4 attenuates the long-term negative effects of a high-saturated fat diet.