Product Name: AMIGO-1
Product Number: AB-NN193-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Amphoterin-induced protein 1
Target Alias: AMIGO 1; AMIGO1; Adhesion molecule with Ig like domain 1; Alivin-2; Alivin 2; Ali2; AMIGO; KIAA1163; Amphoterin induced gene and ORF (Amigo); Amphoterin induced protein 1; MGC25558; OTTHUMP00000013379; RP23 89M15.6
Product Type Specific: Adhesion protein pan-specific antibody
Antibody Code: NN193-1
Antibody Target Type: Pan-specific
Protein UniProt: Q86WK6
Protein SigNET: Q86WK6
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: AMIGO 1; AMIGO1; Adhesion molecule with Ig like domain 1; Alivin-2; Alivin 2; Ali2; AMIGO; KIAA1163; Amphoterin induced gene and ORF (Amigo); Amphoterin induced protein 1; MGC25558; OTTHUMP00000013379; RP23 89M15.6
Product Type Specific: Adhesion protein pan-specific antibody
Antibody Code: NN193-1
Antibody Target Type: Pan-specific
Protein UniProt: Q86WK6
Protein SigNET: Q86WK6
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 554-574 (cytoplasmic C-terminus) of human AMIGO-1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~60-80 kDa protein depending on maturity/glycosylation.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-438 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Potency: In mouse brain lysates, this antibody detects a ~60-80 kDa protein depending on maturity/glycosylation.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-438 was sufficient for detection of AMIGO-1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.
References
[1] Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973.
[2] Clark H.F. et al. (2003) Genome Res. 13: 2265-2270.
[3] On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896.
[4] Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274.
[1] Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973.
[2] Clark H.F. et al. (2003) Genome Res. 13: 2265-2270.
[3] On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896.
[4] Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274.
© Kinexus Bioinformatics Corporation 2017