Product Name: Ankyrin R
Product Number: AB-NN195-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Ankyrin-1
Target Alias: Ankyrin-R; Erythrocyte Ankyrin; ANK-1; ANK; rCG_43073
Product Type Specific: Cytoskeletal protein pan-specific antibody
Antibody Code: NN195-1
Antibody Target Type: Pan-specific
Protein UniProt: P16157
Protein SigNET: P16157
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2B
Antibody Immunogen Source: Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
Production Method: Protein G purified
Target Alias: Ankyrin-R; Erythrocyte Ankyrin; ANK-1; ANK; rCG_43073
Product Type Specific: Cytoskeletal protein pan-specific antibody
Antibody Code: NN195-1
Antibody Target Type: Pan-specific
Protein UniProt: P16157
Protein SigNET: P16157
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2B
Antibody Immunogen Source: Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~200 kDa protein by Western blotting.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~200 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:100 dilution of SMC-487 was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Ankyrin-B or Ankyrin-G.
Related Product 2: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-1)
Related Product 3: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-2)
Related Product 4: Ankyrin-G pan-specific antibody (Cat. No.: AB-NN197-1)
Antibody Positive Control: A 1:100 dilution of SMC-487 was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Ankyrin-B or Ankyrin-G.
Related Product 2: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-1)
Related Product 3: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-2)
Related Product 4: Ankyrin-G pan-specific antibody (Cat. No.: AB-NN197-1)
Scientific Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane. Ankyrin R, or Ank1, was first discovered in erythrocyres, but has since also been located in the brain and muscles. Mutations are associated with hereditary spehrocytosis and alzheimer's diease (2).
References
[1] Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.
[2] Genes and mapped phenotypes. (n.d.). Retrieved April 21, 2015, from http://www.ncbi.nlm.nih.gov/gene/286
[1] Bennett V., Baines A.J. (2001) Physiol. Rev. 81 (3): 1353–92.
[2] Genes and mapped phenotypes. (n.d.). Retrieved April 21, 2015, from http://www.ncbi.nlm.nih.gov/gene/286
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