Product Name: Brevican
Product Number: AB-NN221-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Brevican core protein

Target Alias: BCAN; BEHAB; CSPG7; Brain enriched hyaluronan binding protein; Brevican core protein isoform 1; Brevican core protein isoform 2; Brevican proteoglycan; Chondroitin sulfate proteoglycan 7; Chondroitin sulfate proteoglycan BEHAB; MGC13038

Product Type Specific: Brevican pan-specific antibody

Antibody Code: NN221-1

Antibody Target Type: Pan-specific

Protein UniProt: P55068

Protein SigNET: P55068

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG2B

Antibody Immunogen Source: Fusion protein amino acids 219-655 of rat Brevican

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | ICC/Immunofluorescence

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: In mouse brain lysates, this antibody detects a ~140 kDa protein (and smaller due to proteolytic cleavage).

Antibody Species Reactivity: Mouse | Rat

Antibody Positive Control: 1 µg/ml of SMC-428 was sufficient for detection of Brevican in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Scientific Background: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.