Product Name: CDC37
Product Number: AB-NN230-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Hsp90 co-chaperone Cdc37
Target Alias: CC37; CDC37 (cell division cycle 37,); CDC37 cell division cycle 37; Cell division cycle 37; P50Cdc37
Product Type Specific: CDC37 pan-specific antibody
Antibody Code: NN230-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16543
Protein SigNET: Q16543
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Native human Cdc37, full length
Target Alias: CC37; CDC37 (cell division cycle 37,); CDC37 cell division cycle 37; Cell division cycle 37; P50Cdc37
Product Type Specific: CDC37 pan-specific antibody
Antibody Code: NN230-1
Antibody Target Type: Pan-specific
Protein UniProt: Q16543
Protein SigNET: Q16543
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Native human Cdc37, full length
Production Method: Protein A purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.7 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.7 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~44.5 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human
Antibody Positive Control: A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Species Reactivity: Human
Antibody Positive Control: A 1:2000 dilution of SPC-142 was sufficient for detection of cdc37 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Scientific Background: HSP90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with HSP90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing HSP90 to its target kinases (1, 2). CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies (3, 4).
References
[1] Dai K, Kobayashi R., Beach D. (1996) J Biol Chem. 271(36): 22030-22034.
[2] Stepanova L, Leng X., Parker S.B., Harper J.W. (1996) Genes Dev. 10(12): 1491-1502.
[3] Kimura Y. et al. (1997) Genes Dev. 11(14): 1775-1185.
[4] Gray P.J. et al. (2008) Nat Rev Cancer. 8(7): 491-495.
[1] Dai K, Kobayashi R., Beach D. (1996) J Biol Chem. 271(36): 22030-22034.
[2] Stepanova L, Leng X., Parker S.B., Harper J.W. (1996) Genes Dev. 10(12): 1491-1502.
[3] Kimura Y. et al. (1997) Genes Dev. 11(14): 1775-1185.
[4] Gray P.J. et al. (2008) Nat Rev Cancer. 8(7): 491-495.
© Kinexus Bioinformatics Corporation 2017