Product Name: Chapsyn-110
Product Number: AB-NN232-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Disks large homolog 2
Target Alias: PSD93; Chapsyn-110; MGC131811; PSD-93; Discs Large homologue 2; DLG2; Postsynaptic density protein PSD-93; DLGH2; Channel-associated protein of synapse-110
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN232-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15700
Protein SigNET: Q15700
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 1-852 of rat Chapsyn-110
Target Alias: PSD93; Chapsyn-110; MGC131811; PSD-93; Discs Large homologue 2; DLG2; Postsynaptic density protein PSD-93; DLGH2; Channel-associated protein of synapse-110
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN232-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15700
Protein SigNET: Q15700
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 1-852 of rat Chapsyn-110
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Weak potency. Detects a ~110 kDa protein in cell and tissue lysates by Western blotting. Weak human detection.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-325 was sufficient for detection of Chapsyn -110 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against other MAGUK family members, PSD95, SAP97, SAP102, expressed in transfected cells.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-325 was sufficient for detection of Chapsyn -110 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against other MAGUK family members, PSD95, SAP97, SAP102, expressed in transfected cells.
Scientific Background: Chapsyn-110, is a part of the membrane-associated putative guanylate kinase (MAGUK) family. It binds directly to the NMDA receptor, and Shaker K+ channel subunits, and is 70-80% identical to PSD-95/SAP90 and SAP97 (1). It associated tightly with the postsynaptic density I brain, and mediates the clustering of both NMDA recpetors and K+ channels in heterologous cells. The encoded protein forms a heterodimer with PSD-95 that may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins (1, 2).
References
[1] Kim E. et al. (1996) Neuron. 17:103-113.
[2] Godreau D., Neyroud N., Vranckx R. and Hatem S. (2004) Med Sci (Paris). 20(1): 84-88.
[1] Kim E. et al. (1996) Neuron. 17:103-113.
[2] Godreau D., Neyroud N., Vranckx R. and Hatem S. (2004) Med Sci (Paris). 20(1): 84-88.
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