Product Name: Dicer
Product Number: AB-NN237-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Endoribonuclease Dicer
Target Alias: Dicer1; DCR; DCR1; DCR-1; Double-strand-specific ribonuclease; Helicase with RNase motif; HERNA; Helicase MOI; KIAA0928
Product Type Specific: RNAse pan-specific antibody
Antibody Code: NN237-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UPY3
Protein SigNET: Q9UPY3
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
Target Alias: Dicer1; DCR; DCR1; DCR-1; Double-strand-specific ribonuclease; Helicase with RNase motif; HERNA; Helicase MOI; KIAA0928
Product Type Specific: RNAse pan-specific antibody
Antibody Code: NN237-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UPY3
Protein SigNET: Q9UPY3
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~215 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-416 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
References
[1] Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60.
[2] Lee Y. et al. (2003) Nature 425: 415-9.
[3] Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
[1] Hutvágner G. and Zamore P.D. (2002) Science 297: 2056-60.
[2] Lee Y. et al. (2003) Nature 425: 415-9.
[3] Diederichs S. and Haber, D.A. (2007) Cell 131: 1097-108.
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