Product Name: SAP102
Product Number: AB-NN238-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Disks large homologue 3

Target Alias: DLG3; MRX90; NEDLG; XLMR; PSD-95/SAP90-related protein 1; Synapse-associated protein 102; SAP-102

Product Type Specific: Synaptic protein pan-specific antibody

Antibody Code: NN238-1

Antibody Target Type: Pan-specific

Protein UniProt: Q92796

Protein SigNET: Q92796

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Fusion protein amino acids 1-120 of rat SAP102

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: Medium-high potency. Detects a ~105 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse | Rat

Antibody Positive Control: 1 µg/ml was sufficient for detection of Sap102 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Antibody Specificity: High

Antibody Cross Reactivity: No cross-reactivity against PSD95, SAP97 or Chapsyn-110.

Scientific Background: Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein family and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homology 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with the carboxy-terminal domain of the N-methyl-D-aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth.
References

[1] Muller B.M. et al. (1996) Neuron 17: 255-265.

[2] Lau L. F. et al. (1996) J. Biol. Chem. 271: 21622-21628.

[3] Cuthbert P.C. et al. (2007) J Neuroscience 27:2673-2682.

[4] Lau L.F. et al. (1996) J Biol Chem 274:5782-5790.