Product Name: FIG4/SAC3
Product Number: AB-NN247-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Polyphosphoinositide phosphatase
Target Alias: 5-bisphosphate 5-phosphatase; Fig4; KIAA0274; Sac3; Phosphatidylinositol 3; Phosphatidylinositol 3;5 bisphosphate 5 phosphatase; SAC domain containing protein 3; SAC domain-containing protein 3; SAC3
Product Type Specific: Phosphorylated inositol phosphatase pan-specific antibody
Antibody Code: NN247-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92562
Protein SigNET: Q92562
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: 5-bisphosphate 5-phosphatase; Fig4; KIAA0274; Sac3; Phosphatidylinositol 3; Phosphatidylinositol 3;5 bisphosphate 5 phosphatase; SAC domain containing protein 3; SAC domain-containing protein 3; SAC3
Product Type Specific: Phosphorylated inositol phosphatase pan-specific antibody
Antibody Code: NN247-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92562
Protein SigNET: Q92562
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 688-907 (C-terminus) of mouse Sac3. Rat: 95% identity (203/220 amino acids identical). Human: 91% identity (201/220 amino acids identical)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~100 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-468 was sufficient for detection of FIG4/SAC3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Potency: In mouse brain lysates, this antibody detects a ~100 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-468 was sufficient for detection of FIG4/SAC3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: Fig4/Sac3 phosphatase, a 97-kDa protein, specifically removes the 5-phosphate from the inositol ring of PI(3,5)P2, a membrane-bound phospholipid, which acts as a molecular signal for trafficking and fusion of intracellular vesicles. It is broadly expressed in all eukaryotic cells and plays a vital role in intracellular membrane trafficking and signaling.
Mutations in Fig4/Sac3 have been linked to pale tremor mice that exhibit neuropathy with severe central and peripheral neuronal degeneration, including the loss of neurons in the dorsal root ganglion and the sciatic nerve. Fig4/Sac3 knockout mice have been shown to be smaller in size and exhibit abnormal tremor and gait and neuronal autophagy.
© Kinexus Bioinformatics Corporation 2017