Product Name: FIH
Product Number: AB-NN248-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Hypoxia-inducible factor 1-alpha inhibitor

Target Alias: Factor inhibiting HIF1 (hypoxia-inducible factor); DKFZp762F1811; Factor inhibiting HIF-1; Factor inhibiting HIF1; FIH 1; FIH-1; FIH1; FLJ20615; FLJ22027; HIF1AN; HIF1N_HUMAN; Hypoxia inducible factor 1 alpha subunit inhibitor; Hypoxia inducible factor asparagine hydroxylase; Hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; Peptide aspartate beta dioxygenase

Product Type Specific: Heat shock/stress protein pan-specific antibody

Antibody Code: NN248-1

Antibody Target Type: Pan-specific

Protein UniProt: Q9NWT6

Protein SigNET: Q9NWT6

Antibody Type: Monoclonal
Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Full length human FIH expressed in E.coli BL21 (DE3) cells

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence

Antibody Dilution Recommended: WB (1:100), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Medium potency. Detects a ~45 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse

Antibody Positive Control: 1 µg/ml of SMC-182 was sufficient for detection of FIH in 20 µg of Hela lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Specificity: Medium-low

Scientific Background: FIH, Factor inhibiting HIF1 (hypoxia-inducible factor), is an asparaginyl hydroxylase. FIH in conjunction with VHL represses HIF-1 transcriptional activity by disrupting the interaction of HIF-1 with the transcriptional co-activators CBP/p300, and by recruiting histone deacetylases. FIH activity is inhibited during hypoxia (1-3). Recent studies show that low nuclear expression of FIH is a strong independent prognostic factor for a poor overall survival in clear cell renal cell carcinoma (4).