Product Name: HSP105
Product Number: AB-NN269-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Heat shock protein 105 kDa

Target Alias: Heat shock 105kDa alpha; heat shock 105kDa beta; HSP105; HSP105A; HSP105B; HSP110; HSPH1; NY CO 25

Product Type Specific: Heat shock/stress protein pan-specific antibody

Antibody Code: NN269-1

Antibody Target Type: Pan-specific

Protein UniProt: Q92598

Protein SigNET: Q92598

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Ig Isotype Clone: N/A

Antibody Immunogen Source: Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse

Production Method: Affinity purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry

Antibody Dilution Recommended: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Very high potency. Detects a ~110 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse | Rat | Bovine | Monkey | Hamster | Sheep | Yeast | Mustelus antarcticus (Shark) | Galeorhinus galeus (Shark)

Antibody Positive Control: 1 µg/ml of SPC-195 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Scientific Background: HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).
References

[1] Lee-Yoon D. et al. (1995) J Biol Chem. 270: 15725-15733.

[2] Dragovic Z. et al. (2006) EMBO J. 25: 2519-2528.

[3] Nakamura J. et al. (2008) Stroke. 39(10): 2853-2859.

[4] Subject D.R. (2001) Health Research Inc. Report number AS90893: 1-84.