Product Name: HSPB2 (MKBP)
Product Number: AB-NN270-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Heat shock protein beta-2

Target Alias: DMPK-binding protein; heat shock 27kDa protein 2; HSP27; HSPB2; MKBP; MGC133245

Product Type Specific: Heat shock/stress protein pan-specific antibody

Antibody Code: NN270-1

Antibody Target Type: Pan-specific

Protein UniProt: Q16082

Protein SigNET: Q16082

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Ig Isotype Clone: N/A

Antibody Immunogen Source: Synthetic peptide

Production Method: Affinity purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.02% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | ICC/Immunofluorescence

Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: High potency. Detects a ~27 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse | Rat

Antibody Positive Control: 1 µg/ml of SPC-194 was sufficient for detection of MKBP in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody Specificity: High

Scientific Background: HSPB2, also known as MKBP is the most divergent member of the sHSP family with only 30% sequence identity to all other mammalian sHSPS (1). MKBP is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. MKBP enhances the kinase activity of DMPK and protects it from heat-induced activation. MKBP also shows a unique nature compared to other sHSPs, in that the expression of MKBP is not induced by heat shock (2). In unstressed skeletal muscle, MKBP forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction (1, 3). During stress, these complexes dissolve and MKBP’s localization to mitochondria increases, leading to increased cell survival. Pinz et al. tried to find a distinct role for MKBP in terms of cardiac mechanics and finds that it is required for normal systolic performance and normal cardiac energetic (1). HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, suggesting that MKBP may be an important factor in tumor transformation and metastasis (4).
References

[1] Pinz I. et al. (2008) FASEB J. 22: 84-92.

[2] Suzuki A. et al. (1998) J Cell Bio. 140(5): 1113-1124.

[3] Sugiyama Y. et al. (2000) J Biol Chem. 275: 1095-1104.

[4] Oshita S.E. et al. (2010) Breast Cancer Res Treat. Epub: 20087649.