Product Name: JIP2/IB2
Product Number: AB-NN272-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: C-Jun-amino-terminal kinase-interacting protein 2

Target Alias: MAPK8IP2; C jun amino terminal kinase interacting protein 2; C-jun-amino-terminal kinase-interacting protein 2; Homologous to mouse JIP 1 ; IB 2; IB-2; IB2; Islet brain 2; Islet-brain-2; JIP 2; JIP-2; JIP2; JNK interacting protein 2; JNK MAP kinase scaffold protein 2; JNK MAP kinase scaffold protein JIP2; JNK-interacting protein 2; MAPK8IP2; Mitogen activated protein kinase 8 interacting protein 2; Mitogen-activated protein kinase 8-interacting protein 2; PRKM8 interacting protein like; PRKM8IPL

Product Type Specific: Scaffolding protein pan-specific antibody

Antibody Code: NN272-1

Antibody Target Type: Pan-specific

Protein UniProt: Q13387

Protein SigNET: Q13387
Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Fusion protein amino acids 226-421 of mouse JIP-2/IB-2. Rat: 94% identity (127/135 amino acids identical). Human: 86% identity (173/200 amino acids identical) >50% identity with JIP-1.

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence

Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a >100 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Mouse | Rat

Antibody Positive Control: 1 µg/ml of SMC-465 was sufficient for detection of JIP2/IB2 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Cross Reactivity: Does not cross-react with JIP-1/IB-1.

Scientific Background: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. It is expressed mainly in the cerebellum, pituitary gland, occipital lobe, and the amygdala of the brain, but also in the pancreas, including insulin-secreting cells.