Product Name: KDEL
Product Number: AB-NN277-1
| Size: | 50 µg | Price: | 89.00 | |
| $US |
Target Full Name: Lys Asp Glu Leu peptide
Product Type Specific: KDEL pan-specific antibody
Antibody Code: NN277-1
Antibody Target Type: Pan-specific
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: KDEL containing peptide immunogen
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Product Type Specific: KDEL pan-specific antibody
Antibody Code: NN277-1
Antibody Target Type: Pan-specific
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: KDEL containing peptide immunogen
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a KDEL proteins, GRP94, Grp78, PDI and calreticulin.
Antibody Species Reactivity: Human | Mouse | Rat
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a KDEL proteins, GRP94, Grp78, PDI and calreticulin.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: A 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.
Antibody Cross Reactivity: May also immunoreact with ERp57 and ERp29.
Antibody Cross Reactivity: May also immunoreact with ERp57 and ERp29.
Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
References
[1] Ozawa K. et al. (2008) Mol Pharmacol. 74:1610.
[2] Austin R.C. et al. (2003) J Biol Chem. 278: 17438.
[1] Ozawa K. et al. (2008) Mol Pharmacol. 74:1610.
[2] Austin R.C. et al. (2003) J Biol Chem. 278: 17438.
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