Product Name: MDC1
Product Number: AB-NN289-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Mediator of DNA damage checkpoint protein 1
Target Alias: Nuclear factor with BRCT domains 1
Product Type Specific: MDC1 pan-specific antibody
Antibody Code: NN289-1
Antibody Target Type: Pan-specific
Protein UniProt: Q14676
Protein SigNET: Q14676
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
Target Alias: Nuclear factor with BRCT domains 1
Product Type Specific: MDC1 pan-specific antibody
Antibody Code: NN289-1
Antibody Target Type: Pan-specific
Protein UniProt: Q14676
Protein SigNET: Q14676
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~184 kDa protein by Western blotting. This antibody recognizes MDC1 at and around the N-terminus.
Antibody Species Reactivity: Human | Mouse | Bovine | Chimpanzee
Antibody Positive Control: 0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Very high
Antibody Species Reactivity: Human | Mouse | Bovine | Chimpanzee
Antibody Positive Control: 0.5 µg/ml of SMC-197 was sufficient for detection of MDC1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Very high
Scientific Background: MDC1, mediator of DNA damage checkpoint protein 1, plays a role in checkpoint mediated cell cycle arrest in response to DNA damage, within S phase and G2/M. It is also thought to act as a scaffold protein during recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage that are marked by phosphorylation of histone H2A.X on S139.
References
[1] Lou Z. et al. (2004) J Biol Chem. 279(45): 46359-46362.
[2] Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199.
[3] Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814.
[4] Wilson K.A. et al. (2011) Mol Cancer Res. 9(6): 766-781.
[1] Lou Z. et al. (2004) J Biol Chem. 279(45): 46359-46362.
[2] Luo K., Yuan J, Lou Z. (2011) J Biol Chem. 286(32): 28192-28199.
[3] Strauss C., Halevy T., Macarov M., Argaman L., and Goldbery M. (2011) DNA Repair (Amst.). 10(8): 806-814.
[4] Wilson K.A. et al. (2011) Mol Cancer Res. 9(6): 766-781.
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