Product Name: mGluR1/5
Product Number: AB-NN291-2
Size: 25 µg      Price:89.00
      $US
Target Full Name: Metabotropic glutamate receptor 5

Target Alias: Glutamate receptor metabotropic 5;GPRC1E; GRM 5; GRM5; Metabotropic glutamate receptor 5 variant F; Metabotropic glutamate receptor 5 variant G; Metabotropic glutamate receptor 5 variant H; mGlu5; mGluR5; mGluR5a; mGluR5b; Glutamate receptor metabotropic 1; glutamate receptor; metabotropic 1; GPRC1A; GRM 1; GRM1 alpha; GRM1; GRM1_HUMAN; GRM1A; Metabotropic glutamate receptor 1; mGlu1; mGluR1 alpha; mGluR1; mGluR1A

Product Type Specific: G protein-coupled receptor pan-specific antibody

Antibody Code: NN291-2

Antibody Target Type: Pan-specific

Protein UniProt: P41594

Protein SigNET: P41594

Antibody Type: Monoclonal
Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation

Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a ~130 kDa protein (both mGluR1 and mGluR5).

Antibody Species Reactivity: Human | Mouse | Rat

Antibody Positive Control: 1 µg/ml of SMC-407 was sufficient for detection of mGluR1/5 glutamate receptor in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Related Product 1: mGluR1/5 glutamate receptor pan-specific antibody (Cat. No.: AB-NN291-1)

Scientific Background: The AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the CNS. AMPARs are composed of four types of subunits, designated as GluR1, GluR2, GluR3 and GluR4, which combine to form tetramers (1,2).