Product Name: MMP9
Product Number: AB-NN292-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Matrix metalloproteinase-9
Target Alias: MMP-9; CLG4B; 82kDa matrix metalloproteinase-9; collagenease type 4 beta; GELB ; Macrophage gelatinase; MANDP2; Type V collagenase; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B
Product Type Specific: Mettaloproteinase pan-specific antibody
Antibody Code: NN292-1
Antibody Target Type: Pan-specific
Protein UniProt: P14780
Protein SigNET: P14780
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Target Alias: MMP-9; CLG4B; 82kDa matrix metalloproteinase-9; collagenease type 4 beta; GELB ; Macrophage gelatinase; MANDP2; Type V collagenase; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B
Product Type Specific: Mettaloproteinase pan-specific antibody
Antibody Code: NN292-1
Antibody Target Type: Pan-specific
Protein UniProt: P14780
Protein SigNET: P14780
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein amino acids 1-708 (full length) of rat MMP9
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation | FCM
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation | FCM
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~92 kDa protein and ~82 kDa protein (pro and active forms).
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-396 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Potency: Detects a ~92 kDa protein and ~82 kDa protein (pro and active forms).
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-396 was sufficient for detection of MMP9 in 20 µg of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Scientific Background: MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes like arthritis and metastasis (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).
References
[1] Hirose Y. et al. (2008) Am J Hum Genet. 82(5): 1122-1129.
[2] Coussens L.M. et al. (2002) Science 295: 2387-2391.
[3] Sternlicht M.D. et al. (1999) Cell 98: 137-146.
[4] Vu T.H. et al. (1998) Cell 93: 411-422.
[5] Nagase H. et al. (1990) Biochemistry 29: 5783-5789.
[1] Hirose Y. et al. (2008) Am J Hum Genet. 82(5): 1122-1129.
[2] Coussens L.M. et al. (2002) Science 295: 2387-2391.
[3] Sternlicht M.D. et al. (1999) Cell 98: 137-146.
[4] Vu T.H. et al. (1998) Cell 93: 411-422.
[5] Nagase H. et al. (1990) Biochemistry 29: 5783-5789.
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