Product Name: Neuroligin 3
Product Number: AB-NN295-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Neuroligin-3
Target Alias: Gliotactin homologue; Nlgn3; NLGN3_HUMAN
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN295-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9NZ94
Protein SigNET: Q9NZ94
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Target Alias: Gliotactin homologue; Nlgn3; NLGN3_HUMAN
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN295-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9NZ94
Protein SigNET: Q9NZ94
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3. Mouse: 99% identity (118/119 amino acids identical). Human: 98% identity (116/119 amino acids identical) ~60% identity with Neuroligin-1. ~40% identity with Neuroligin-2.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~110 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-471 was sufficient for detection of Neuroligin 3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~110 kDa protein by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-471 was sufficient for detection of Neuroligin 3 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: Does not cross-react with Neuroligin-1, -2, -4 or -4. Weak~120 kDa target protein and one very strong ~75 kDa cross-reactive protein in mouse brain lysates.
Related Product 1: Neuroligin 1 pan-specific antibody (Cat. No.: AB-NN294-1)
Related Product 2: Neuroligin 4 pan-specific antibody (Cat. No.: AB-NN296-1)
Related Product 1: Neuroligin 1 pan-specific antibody (Cat. No.: AB-NN294-1)
Related Product 2: Neuroligin 4 pan-specific antibody (Cat. No.: AB-NN296-1)
Scientific Background: Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses.
© Kinexus Bioinformatics Corporation 2017