Product Name: Notch1
Product Number: AB-NN298-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Neurogenic locus notch homologue protein 1
Target Alias: hN1; Translocation associated notch protein; TAN1; TAN-1; Motch A; mT14; p300; Motch; Mis6; Neurogenic locus notch protein homologue; NICD; Notch 1 intracellular domain; Notch gene homologue 1 (Drosophila); Notch homologue 1; translocation-associated (Drosophila); NOTCH Drosophila; homologue of 1; Translocation associated notch homologue; Translocation associated notch protein TAN 1; xotch
Product Type Specific: Notch pan-specific antibody
Antibody Code: NN298-1
Antibody Target Type: Pan-specific
Protein UniProt: P46531
Protein SigNET: P46531
Antibody Type: Monoclonal
Target Alias: hN1; Translocation associated notch protein; TAN1; TAN-1; Motch A; mT14; p300; Motch; Mis6; Neurogenic locus notch protein homologue; NICD; Notch 1 intracellular domain; Notch gene homologue 1 (Drosophila); Notch homologue 1; translocation-associated (Drosophila); NOTCH Drosophila; homologue of 1; Translocation associated notch homologue; Translocation associated notch protein TAN 1; xotch
Product Type Specific: Notch pan-specific antibody
Antibody Code: NN298-1
Antibody Target Type: Pan-specific
Protein UniProt: P46531
Protein SigNET: P46531
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a >270 kDa protein, ~120 kDa protein and small fragments due to proteolysis.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Notch 2 or Notch3.
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a >270 kDa protein, ~120 kDa protein and small fragments due to proteolysis.
Antibody Species Reactivity: Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with Notch 2 or Notch3.
Scientific Background: Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
References
[1] Swiatek P.J. et al. (1994) Genes Dev. 8: 707-719.
[2] Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company.
[3] Lindsell C.E. et al. (1995) Cell 80: 909-917.
[4] Uyttendaele H. et al. (1996) Development 122: 2251-2259.
[5] Girard L. et al. (1996) Genes Dev. 10: 1930-1944.
[1] Swiatek P.J. et al. (1994) Genes Dev. 8: 707-719.
[2] Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company.
[3] Lindsell C.E. et al. (1995) Cell 80: 909-917.
[4] Uyttendaele H. et al. (1996) Development 122: 2251-2259.
[5] Girard L. et al. (1996) Genes Dev. 10: 1930-1944.
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