Product Name: Piccolo
Product Number: AB-NN307-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Piccolo
Target Alias: ACZ; Aczonin; PCLO; Pico; presynaptic cytomatrix protein
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN307-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y6V0
Protein SigNET: Q9Y6V0
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2b Kappa
Antibody Immunogen Source: Full length protein, BC001304.1
Production Method: Protein G purified
Target Alias: ACZ; Aczonin; PCLO; Pico; presynaptic cytomatrix protein
Product Type Specific: Synaptic protein pan-specific antibody
Antibody Code: NN307-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9Y6V0
Protein SigNET: Q9Y6V0
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2b Kappa
Antibody Immunogen Source: Full length protein, BC001304.1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ELISA
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-weak potency. Detects a ~550 kDa protein in cell and tissue lysates by Western blotting.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ELISA
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-weak potency. Detects a ~550 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No cross-reactivity to Bassoon.
Related Product 1: Piccolo pan-specific antibody (Cat. No.: AB-NN307-2)
Antibody Positive Control: 1 µg/ml of SMC-188 was sufficient for detection of Piccolo in 20 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: No cross-reactivity to Bassoon.
Related Product 1: Piccolo pan-specific antibody (Cat. No.: AB-NN307-2)
Scientific Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
References
[1] Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619.
[2] Wang X. et al. (1999) J Cell Biol. 147(1): 151-162.
[3] Dresbach T. et al. (2006) J Biol Chem. 281(9):6038-6047.
[1] Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619.
[2] Wang X. et al. (1999) J Cell Biol. 147(1): 151-162.
[3] Dresbach T. et al. (2006) J Biol Chem. 281(9):6038-6047.
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