Product Name: SHANK2
Product Number: AB-NN318-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: SH3 and multiple ankyrin repeat domains protein 2

Target Alias: Shank2; PROSAP1; cortactin binding protein 1; KIAA1022; AUTS17; CortBP1; Proline-rich synapse-associated protein 1; Cortactin-binding protein 1; proline-rich synapse associated protein 1; SPANK-3; GKAP/SAPAP interacting protein; SHANK; cortactin SH3 domain-binding protein; ProSAP1; CORTBP1CTTNBP1; SH3 and multiple ankyrin repeat domains 2

Product Type Specific: Synaptic protein pan-specific antibody

Antibody Code: NN318-1

Antibody Target Type: Pan-specific

Protein UniProt: Q9UPX8

Protein SigNET: Q9UPX8

Antibody Type: Monoclonal

Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (SH3 and multiple ankyrin repeat domains protein 2)

Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation

Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody Potency: Detects a ~160 kDa protein in cell and tissue lysates by Western blotting. Weak mouse detection.

Antibody Species Reactivity: Human | Mouse | Rat

Antibody Positive Control: 1 µg/ml of SMC-328 was sufficient for detection of Shank2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Antibody Cross Reactivity: No cross-reactivity against Shank1 or Shank3.

Related Product 1: SHANK1/SHANK3 pan-specific antibody (Cat. No.: AB-NN319-1)

Scientific Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer’s disease (3).
References

[1] Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856.

[2] Kim J.H. et al. (2009) Exp Mol Med. 41(4): 236-242.

[3] Gong Y. et al. (2009) Brain Res. 1292: 191-198.