Product Name: NHE3
Product Number: AB-NN326-1
| Size: | 25 µg | Price: | 89.00 | |
| $US |
Target Full Name: Sodium/hydrogen exchanger 3
Target Alias: SLC9A3; Na+/H+ exchanger 3; isoform 3; Solute carrier family 9 member 3; NHE3MGC126720; MGC126718; solute carrier family 9 (sodium/hydrogen exchanger) member 3; NHE-3; Na(+)/H(+) exchanger 3
Product Type Specific: Solute carrier protein pan-specific antibody
Antibody Code: NN326-1
Antibody Target Type: Pan-specific
Protein UniProt: P48764
Protein SigNET: P48764
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Target Alias: SLC9A3; Na+/H+ exchanger 3; isoform 3; Solute carrier family 9 member 3; NHE3MGC126720; MGC126718; solute carrier family 9 (sodium/hydrogen exchanger) member 3; NHE-3; Na(+)/H(+) exchanger 3
Product Type Specific: Solute carrier protein pan-specific antibody
Antibody Code: NN326-1
Antibody Target Type: Pan-specific
Protein UniProt: P48764
Protein SigNET: P48764
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide mapping to AA 809 to 831 of rat sequence
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~84 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Rat | Mouse
Antibody Positive Control: 1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Potency: Detects a ~84 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Rat | Mouse
Antibody Positive Control: 1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Scientific Background: Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
References
[1] Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942.
[2] Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
[1] Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942.
[2] Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
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