Product Name: SNAP-25
Product Number: AB-NN327-1
Size: 25 µg      Price:89.00
      $US
Target Full Name: Synaptosomal-associated protein 25

Target Alias: Bdr; RIC4; SNAP25; SNAP-25B; SNP25; SUP; super protein; synaptosomal associated 25kDa protein

Product Type Specific: Synaptic protein pan-specific antibody

Antibody Code: NN327-1

Antibody Target Type: Pan-specific

Protein UniProt: P60880

Protein SigNET: P60880

Antibody Type: Monoclonal

Antibody Host Species: Mouse

Antibody Ig Isotype Clone: IgG1

Antibody Immunogen Source: Raised against a crude synaptic preparation from the post mortem human brain
Production Method: Protein G purified

Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | ELISA

Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:1000) ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~29 kDa protein in cell and tissue lysates by Western blotting.

Antibody Species Reactivity: Human | Mouse | Rat | Cat | Pig

Antibody Positive Control: 1 µg/ml of SMC-180 was sufficient for detection of SNAP-25 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Antibody Specificity: Very high

Scientific Background: Synaptosomal-associated protein 25 (SNAP-25) is a membrane bound protein anchored to the cytosolic face of membrane via palmitoyl side chains in the middle of the molecule (1). It is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together (2). SNAP25 exists as two alternatively spliced isoforms, SMAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein (3).